THE INFLUENCE OF Na-ASCORBATE ON CHANGING THE ILEUM SENSITIVITY

In the case of previously mentioned Hartley guinea pigs, male gender, weight from 250 to 300 g, which lived under normal lab’s conditions, The effect of different Na-ascorbate concentrations was studied. Various Na-ascorbate concentrations were used starting from 10-6M to 10-1M, while the sensitivity of terminal part of ileum was determined by Shultz-Dale’s reaction.The samples of ileum treated by 10-5M histamin and the induced contraction served as a maximal response (100%). The ileum sample was rinsed by Thyrode’s solution, the media was exchanged by different concentrations of Na-ascorbate, and with the help of Shulzt-Dale’s reaction, the intensity of ileum contraction was determined.The obtained results show that, with the increase of Na-ascorbate concentration from 10-6M till 10-3M, there is a mild increase of histamin concentration in order to get 50% of response.However, after the applied vitamin C concentration of 10-3 M, the need for histamine suddenly increases in order to get 50% of response, and it achieves its maximum after the 10-1M Na-ascorbate concentration has been applied.These results show that the application of Na-ascorbate lessens the sensitivity of termal part of ileum.