Research: ISOLATION OF RUTIN FROM Ruta graveolens (RUTACEAE) CULTIVATED IN IRAQ BY PRECIPITATION AND FRACTIONAL SOLUBILIZATION

Ruta graveolens L., a plant belongs to the family Rutaceae, is characterized by a particularly wide spectrum ofdifferent plant constituents. More than 100 compounds have been indentified, these belong to four major classesof substances namely alkaloids, coumarins, flavonoids, and essential oils. Rutin is the major flavonoid glycosidefound in Ruta graveolens is the rhamnoglucoside of the flavonoid quercetin has been called vitamin P or thepermeability factor. Several studies had been done for the isolation of rutin by different chromatographic method.In this study rutin was isolated from Ruta graveolens by precipitation and fractional solubilization without the useof any chromatographic technique. The isolated rutin was identified by measuring its melting point, UV absorption,FTIR spectra, HPLC and TLC.