Research: MICROWAVE ASSISTED EXTRACTION AND ESTIMATION OF PIPERINE, ANDROGRAPHOLIDE USING HPLC TECHNIQUES

Plants belonging to the genus piper are reputed in the Indian ayurvedic system of medicine for their medicinal properties. Andrographis paniculata, commonly as "King of Bitters” is a member of the plant family Acanthaceae. Research design: Indian herbal pharmacopoeia prescribes the extraction of piperine for 1 h and 3 h for andrographolide by reflux method followed by quantitative estimation using HPLC technique. In the present study, the extraction procedure of the above said phyto-constituents was planned to develop under microwave condition. Methods and Procedures: The method was focused for successful extraction with less volume of solvent, shorter duration and more yield than the reflux method. Several intensities and durations under microwave conditions were tried. Main outcomes and results: The higher yield of 2.12 % (at 500 W intensity & 16 min) of piperine and 0.20 % of andrographolide (at 500 W intensity & 21 min) were obtained in green technique as compared with the yield of 2.02 % of piperine and 0.18 % of andrographolide obtained by conventional technique. The novel green technique can be used in academics and industries for the extraction of phytoconstituents in comparatively very shorter duration with less consumption of solvents.