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MyD88-dependent and independent pathways of Toll-Like Receptors are engaged in biological activity of Triptolide in ligand-stimulated macrophages

DOI: 10.1186/1472-6769-10-3

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Abstract:

Here we report the expression profile of immune signaling genes modulated by triptolide in LPS induced mouse macrophages. In an array study triptolide treatment modulated expression of 22.5% of one hundred and ninety five immune signaling genes that included Toll-like receptors (TLRs). TLRs elicit immune responses through their coupling with intracellular adaptor molecules, MyD88 and TRIF. Although it is known that triptolide inhibits NFκB activation and other signaling pathways downstream of TLRs, involvement of TLR cascade in triptolide activity was not reported. In this study, we show that triptolide suppresses expression of proinflammatory downstream effectors induced specifically by different TLR agonists. Also, the suppressive effect of triptolide on TLR-induced NFκB activation was observed when either MyD88 or TRIF was knocked out, confirming that both MyD88 and TRIF mediated NFκB activation may be inhibited by triptolide. Within the TLR cascade triptolide downregulates TLR4 and TRIF proteins.This study reveals involvement of TLR signaling in triptolide activity and further increases understanding of how triptolide activity may downregulate NFκB activation during inflammatory conditions.Chronic inflammation is an important patho-physiological condition impacting various diseases including rheumatoid arthritis (RA), atherosclerosis, diabetes, and cancer. Recent evidence suggests the involvement of Toll-like receptors (TLRs) in various chronic inflammatory and autoimmune diseases [1-3]. TLRs belong to the family of pathogen-associated molecular pattern recognition receptors and are vital components of the host's immune system for sensing dangerous pathogens, and for initiating inflammatory and immune responses directed against these pathogens. The mechanism of signal transduction through TLRs is well characterized [4-11]. There are two possible routes for mediation of signals received by TLRs depending on which of the two adapter molecules (MyD88 and TRIF) are

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