IDENTIFICATION AND CHARACTERIZATION OF NEUTRAL PROTEASE PRODUCING Paenibacillus Polymyxa SPECIES EMBS024 BY 16S rRNA GENE SEQUENCING

The term probiotics means supporting life, just being on the opposite spectrum to antibiotic which is against life. Probiotic microorganisms are those microbially derived factors that stimulate the growth of other microorganisms. They are considered to be the best alternative of antibiotics. The current study illustrate the identification of a neutral protease producing bacteria from soil by 16s rRNA sequencing technique. The sample was collected from outskirts of Vijayawada, Andhra Pradesh, India, over dump food wastes soil. The collected sample was serially diluted and the targeted colony after incubation over a certain time period was introduced to 16s rRNA sequencing. The result showed the extract to be a neutral protease producing bacteria which was further named as Paenibacillus polymyxa strain EMBS024. After characterization the sequence of isolate was deposited in GenBank with accession number JQ004091.