TESTING OF TWO PROTOCOLS FOR GENOTYPING THE LEPTIN GENE LOCUS AND BODY MEASUREMENT IN MARAMURES BROWN AND ROMANIAN BREED CATTLE

The body measurement of Maramures Brown breed and Romanian Siemmental and testing twoprotocols for emphasizing the leptine gene in order to perform associations with some beef productiontraits within further studies were the aims of our research. The blood DNA extraction was performedaccording to the protocols proposed by Yves Amigues and genotyping protocols was proposed byLeifers and Pomp et al. The body weight is within standards. The analyzed protocols used for leptinegene emphasizing led to satisfactory results, which will enable us to perform further research in orderto make associations between this possible marker gene and some body traits.