Computer Assisted Semen Analysis of Epididymal Spermatozoa after an Interperitoneal Administration of Diazinon and Cadmium

The effects of cadmium and diazinon administration on rat sperm motility parameters were performed using a Computer Assisted Semen Analyzer (CASA) system. CASA was made on semen samples collected 36 h following an intraperitoneal injection of physiological solution (control group), diazinon (20 mg/kg body wt), cadmium (2 mg/kg body wt), separately and in combination. Sperm kinematic measurements revealed significant decline in the percentage of motile spermatozoa (P<0.05), significant higher amplitude of lateral head displacement (ALH) (P<0.01) with significant decline in beat cross frequency (BCF) (P<0.001) after the diazinon administration. Cadmium-treated males exhibited significantly lower percentage of motile spermatozoa (P<0.01) and percentage of spermatozoa with progressive motility (P<0.001) with significant decrease in straightness (STR) and BCF (P<0.05). Simultaneous exposure to cadmium and diazinon led to significant increase in ALH (P<0.05) and significant decrease in BCF (P<0.001). Cadmium and diazinon exerted deleterious effect inducing spermatozoa motility alterations which could be subsequently negatively related to male fertility.