Actinomycetes FROM HOSPITAL DUMP SOIL PRODUCE HIGHLY ACTIVE ANTIBIOTIC

Antibiotic producing actinomycetes can be identified from soil from different locations including various geographical locations. But antibiotic producing actinomycetes are yet to be identified from hospital wasteland soil or drainage soil. In this study, we have identified 11 actinomycetes isolate from five different hospital wasteland and drainage soil of West Bengal, India. Only 1 isolate out of 11 isolates is found to be antibiotic producer and this antibiotic is showing high level of antibacterial activity against both gram positive and gram negative microorganisms. The crude extract of the antibiotic is found to be more active against gram positive microorganisms whereas ethyl acetate fractionated antibiotics is showing almost two fold more activity than its crude counterpart against gram negative microorganisms. Ethyl acetate fractionated antibiotic is demonstrating more activies than standard antibiotic, chloramphenicol against all the test microorganisms. MIC of the antibiotic is estimated to be 18μg against Bacillus cereus. Ethyl acetate fractionated antibiotic shows a single band on TLC plate.