In vitro determination of anti-oxidant activities of Garuga pinnata Roxb

Medicinal plants are alternative source for the anti-oxidants which don’t possess side effects and more potent than synthetic drugs. The present investigation was carried out to evaluate anti-oxidant properties of Garuga pinnata leaves, stem bark and fruit which are extracted with methanol. The anti-oxidant properties of these plant extracts of various concentrations (50, 150 and 250 μg/ml) was determined by using various methods like DPPH radical scavenging, hydroxyl radical scavenging, superoxide anion, hydrogen peroxide and nitrous oxide the results are compared with known standards. All the plant extracts exhibited notable inhibition of free radicals generation at all concentrations tested. The highest scavenging activity was shown by stem bark extract followed by leaf and fruit. A systemic investigation was also carried out to evaluate to identify the total phenolics and flavanoid to explore relation with anti-oxidant properties.