Primary vaccination evaluation against equine influenza virus (H3N8) in Morocco

The present work is to evaluate the humoral response provided by two injections of primary vaccination (day 0 and day 28) recombinant vaccine in 31 seronegative equids against equine influenza (9 horses, 16 donkeys and 6 mules), of which eight sets of blood samples were performed weekly. After centrifugation, the sera obtained were analyzed by the hemagglutination inhibition test. The results of the follow of the variation in antibodies showed that the primary vaccination of recombinant vaccine provides a protective humoral response against equine influenza for the three species studied. The protective antibody levels well above the range of vaccine protection adopted by several authors (1 / 20, 1 / 40). Antibody production exceeds the title of 1 / 20 (> 4log2) in less than a week and to the maximum immunity from the 14 to 21 day of the first injection of the primary vaccination. The mules have increased antibody titers of 2 log2 after the second injection. For all equines tested the antibody titer remained high two months after primary vaccination (1/64 or (8 log 2)).