COMPARAISON DE DEUX TECHNIQUES IMMUNOENZYMATIQUES ELISA POUR LA DETECTION DES ANTICORPS IgG SERIQUES ANTIRUBEOLIQUES

OBJECTIVE: We evaluate the technical characteristics of two immunoassays (ELISA principle, performance and limitations) used for the detection of serum rubella IgG. MATERIAL AND METHODS: The search for rubella IgG was performed in 100 serum samples collected in the laboratory of immunoserology in the CHU Ibn Rochd of Casablanca. They come from patients and consultants of the University Hospital. They are mostly young women (85%) and newborns (12%). The immunoassays methods used are: 1 - Kit Vircell Spain (manual technique) 2 - AxsymAbbott USA (technique MEIA automated). RESULTS: The serological results for the detection of rubella IgG antibodies have shown a concordance between the two techniques of 94% (90% positive results and 4% negative), and a discrepancy of 6%. CONCLUSION: Although the correlation between the two techniques is 94%, discordant results demonstrate the need to always integrate the approach diagnostic of a clinical data associated with biological data, and to take into account the threshold of detection of serological markers of the specific technique used, and the quality of the samples tested.