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BMC Cancer  2008 

ERCC1 and BRCA1 mRNA expression levels in metastatic malignant effusions is associated with chemosensitivity to cisplatin and/or docetaxel

DOI: 10.1186/1471-2407-8-97

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Abstract:

Real-time quantitative PCR is used to analysis related genes expression in forty-six malignant effusions prospectively collected from non-small cell lung cancer (NSCLC), gastric and gynecology cancer patients. Viable tumor cells obtained from malignant effusions are tested for their sensitivity to cisplatin and docetaxel using ATP-TCA assay.ERCC1 expression level is negatively correlated with the sensitivity to cisplatin in NSCLC patients (P = 0.001). In NSCLC and gastric group, BRCA1 expression level is negatively correlated with the sensitivity to cisplatin (NSCLC: P = 0.014; gastric: P = 0.002) while positively correlated with sensitivity to docetaxel (NSCLC: P = 0.008; gastric: P = 0.032). A significant interaction is found between ERCC1 and BRCA1 mRNA expressions on sensitivity to cisplatin (P = 0.010, n = 45).Our results demonstrate that ERCC1 and BRCA1 mRNA expression levels are correlated with in vitro chemosensitivity to cisplatin and/or docetaxel in malignant effusions of NSCLC and gastric cancer patients. And combination of ERCC1 and BRCA1 may have a better role on predicting the sensitivity to cisplatin than the single one is considered.The lack of understanding and accounting for inter-patient variability in drug efficacy is one of the handicaps in the field of chemotherapy, which inevitably leads to the unpredictable disease responses and patient toxicities. New advanced technology, such as phamacogenomics, offers us a more efficient tool to explore the candidate genes that influence drug activity and toxicity. Such studies make it possible to perform tailor chemotherapy based on the specific genetic profile of individual patients [1,2]. Moreover, establishing any potent biomarkers for chemotherapy resistance will allow for the adoption of a better chemotherapeutic regimen, which will maximize efficacies and minimize toxicities of chemotherapy.Genetic alterations are one of the major reasons for chemo-resistant in metastases[3]. Given the complicated b

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