Anti-proliferative effects of Bifidobacterium adolescentis SPM0212 extract on human colon cancer cell lines

The anti-proliferative activity of B. adolescentis isolates was assessed by XTT assays on three human colon cancer cell lines (Caco-2, HT-29, and SW480). The effects of B. adolescentis SPM0212 butanol extract on tumor necrosis factor-α (TNF-α) and nitric oxide (NO) production were tested using the murine macrophage RAW 264.7 cell line.The butanol extract of B. adolescentis SPM0212 dose-dependently inhibited the growth of Caco-2, HT-29, and SW480 cells by 70%, 30%, and 40%, respectively, at 200 μg/mL. Additionally, the butanol extract of B. adolescentis SPM0212 induced macrophage activation and significantly increased the production of TNF-α and NO, which regulate immune modulation and are cytotoxic to tumor cells.The butanol extract of B. adolescentis SPM0212 increased activity of the host immune system and may improve human health by helping to prevent colon cancer as a biological response modifier.Colon cancer is a serious health problem and remains the leading cause of cancer mortality throughout the world [1]. Colon cancer incidence has rapidly increased as dietary patterns have changed to contain high fat, high protein, low carbohydrate, and low fiber [2,3]. Colon cancer is the second most common cancer in Korea [4]. Despite recent advances in our understanding of the biological processes resulting in the development of cancer, there remains a need for new and effective agents to control this disease.Microorganisms, such as Mycobacterium bovis, Streptococcus pyogenes, Corynebacterium parvum, and cellular components of these bacteria have been used as biological response modifiers (BRM) and are beneficial adjuvants to cancer chemotherapy, increasing remission rates and disease-free intervals. However, the side effect profile in clinical applications for human cancer therapy is important, as these bacteria are pathogens [5-7].The health and nutritional benefits of orally administered probiotic lactic acid bacteria, such as Lactobacillus and Bifidobacteria species