Aspectos morfofisiológicos na pré-aclimatiza o in vitro e aclimatiza o de plantas de caroá Morpho-physiological aspects of in vitro pre-acclimatization and acclimatization of the caroá plant

O objetivo deste trabalho foi estudar a influência da sacarose (0; 15 e 30 g L-1), tipo de veda o (filme de PVC e tamp o de algod o) dos tubos de ensaio, e a concentra o dos sais do meio MS (50; 75 e 100%) sobre os aspectos morfofisiológicos das plantas de caroá durante a fase de pré-aclimatiza o in vitro visando otimizar a taxa de sobrevivência e reduzir o tempo de aclimatiza o das mudas. A análise das características de comprimento, produ o de matéria seca, taxa de sobrevivência e enraizamento mostraram que as plantas de Neoglaziovia variegata nas fases de pré-aclimatiza o in vitro e aclimatiza o foram dependentes das concentra es de sacarose e nutrientes inorganicos, além da aera o. Melhores resultados foram obtidos quando as mudas de caroá foram inoculadas em meio MS com a concentra o original dos sais do MS, suplementado com 15 ou 30 g L-1 de sacarose e vedadas com tamp o de algod o. This work aimed to evaluate the influence of sucrose (0; 15 and 30g L-1), the type of seal used in the test tubes (PVC film or cotton plug), and salt concentration in the MS medium (50%; 75% and 100%) on the morphological and physiological traits of caroá plants during in vitro pre-acclimatization phase aiming to optimize the survival rate and shorten the acclimatization period of the seedlings. The analysis of characteristics such as length, dry matter production, survival rate and rooting, showed that Neoglaziovia variegata plants at pre-acclimatization and acclimatization stages were dependent on sucrose concentrations, inorganic nutrients from MS medium and aeration. The best results in the acclimatization stage were obtained when the caroá seedlings were inoculated in MS medium with the full salt concentration, supplemented with 15 or 30g L-1 sucrose, and sealed with cotton plugs during the pre-acclimatization stage.