Evaluation of Phytochemical and Anti-Microbial Activity of Ethanolic Extract of Limonia Acidissima L. Leaves

Herbal medicines as the major remedy in traditional system of medicine have been used in medical practices since antiquity. In addition to its ancient historical uses, wood apple is used in several systems of medicine for a variety of ailments. The objective of the present study was to investigate the presence of phytochemicals from the ethanolic extract of Limonia acidissima L. leaves and their anti-bacterial action against various Gram-positive and Gram-negative bacteria. The potential antibacterial activity against bacteria was examined by minimum inhibitory concentration and zone of inhibition analysis. Minimum inhibitory concentration values compared with control and zone of inhibition values compared with standard ciprofloxacin. The results revealed that, the ethanolic extract is potent in inhibiting bacterial growth of both gram negative and gram-positive bacteria and comparable with the standard (ciprofloxacin). The 200 μg/ml of ethanolic extract showed the best antibacterial activity as compared to the other concentrations. Hence, this plant can be further subjected to isolation of the therapeutic antibacterials and further pharmacological evaluation.