METHYLENETETRAHYDROFOLATE REDUCTASE GENE (677C→T) AND TUMOR NECROSIS FACTOR ALPHA (TNF-α) ASSOCIATED RISK FOR THE DEVELOPMENT OF GESTATIONAL TROPHOBLASTIC NEOPLASIA

Gestational trophoblastic neoplasia is a rare disease of female reproductive system occurs due to aggressiveness of trophoblastic cells start of invade into endometrium or myometrium results profusely bleeding with pain, if fail to cure early the “risk” for mortality may enhanced. The etiology of gestational trophoblastic neoplasia is still unknown but believes to be the interaction between gene and environment. RFLP analysis of MTHFR (C677T) gene showing 28.00% in CT (heterozygous) genotype in patients or cases as comparison to controls (10.00%), suggesting increase “risk factor” in such patients. However, the TNF-α gene showing variable frequency mutation i.e. over expression (57.00%) and down regulation (28.00%) were observed in cases. The odd ratio was also calculated at 95% C.I. for TNF-α gene which reveals highly significant (p=0.009) difference between cases and controls, suggest confirmation of gene-gene interaction in the patients of gestational trophoblastic neoplasia reporting first time in India.