Effect of Substitution of Buffalo Bull Seminal Plasma with That of Cow Bull on Liveability and Conception Rate

The present project was designed to study the effect of substitution of buffalo bull seminal plasma with that of cow bull on liveability and fertility of buffalo bull spermatozoa. After collection, semen was divided into three fractions. a) Half of the seminal plasma of buffalo bull was substituted with equal amount of cow bull seminal plasma, b) The seminal plasma of buffalo bull was completely substituted with that of cow bull, c) No substitution was made (control group). The liveability (hrs) of buffalo bull spermatozoa stored at 37°C was significantly higher (P<0.05) in half substituted seminal plasma (21.3) as compared to that in full substituted (8.4) and control groups (l3.7). Significantly higher conception rate was observed with half substituted seminal plasma semen samples (65.58) as compared to inseminations with control group (54.4) semen samples.