Hypothalamic Expression of KiSS1 and RFamide-related Peptide-3 mRNAs during The Estrous Cycle of Rats

Kisspeptin and RFamide-related peptide-3 (RFRP-3) are known to affect GnRH/luteinizinghormone (LH) in several species, including the rat. It has been hypothesized that GnRH/LH changesduring the rat estrous cycle may result from changes in the expression of KiSS1 and RFRP-3 genes.Therefore, the present study investigates KiSS1 and RFRP-3 gene expression at the transcriptionallevel in the rat hypothalamus during the estrous cycle.In the present experimental study, 36 adult female Sprague-Dawley rats (3-4 months old) were used tostudy the expression of KiSS1 and RFRP-3 mRNA in the hypothalamus during the estrous cycle. Fourrats were ovariectomized, whereas the remainder were allotted to four different phases of the estrouscycle (n=8 per estrus phase). Rats were decapitated, and the hypothalami were immediately dissected andfrozen in liquid nitrogen. Expressions of KiSS1 and RFRP-3 mRNAs were analyzed by real-time PCR.The expression of KiSS1 mRNA during estrus was lower than other phases of the cycle (p<0.01).Expression of KiSS1 mRNA during the metestrus phase was lower than the proestrus phase (p<0.01).The expression of RFRP-3 mRNA during proestrus was lower than the diestrus phase (p<0.01).Results of the present study showed the role of coordinated expression of KiSS1 and RFRP-3 mRNAin the hypothalamus in the control of the rat estrous cycle.