Reconstruction of metabolic pathways for the cattle genome

An amalgamated cattle genome database was created using the NCBI and Ensembl cattle genome databases (based on build 3.1) as data sources. PathwayTools was used to create a cattle-specific pathway genome database, which was followed by comprehensive manual curation for the reconstruction of metabolic pathways. The curated database, CattleCyc 1.0, consists of 217 metabolic pathways. A total of 64 mammalian-specific metabolic pathways were modified from the reference pathways in MetaCyc, and two pathways previously identified but missing from MetaCyc were added. Comparative analysis of metabolic pathways revealed the absence of mammalian genes for 22 metabolic enzymes whose activity was reported in the literature. We also identified six human metabolic protein-coding genes for which the cattle ortholog is missing from the sequence assembly.CattleCyc is a powerful tool for understanding the biology of ruminants and other cetartiodactyl species. In addition, the approach used to develop CattleCyc provides a framework for the metabolic reconstruction of other newly sequenced mammalian genomes. It is clear that metabolic pathway analysis strongly reflects the quality of the underlying genome annotations. Thus, having well-annotated genomes from many mammalian species hosted in BioCyc will facilitate the comparative analysis of metabolic pathways among different species and a systems approach to comparative physiology.Production of domesticated cattle (Bos taurus and Bos indicus) accounts for 7% of the total food consumption in the world [1] and contributes 17.0% of all farm cash receipts in the United States [2]. Thus, there has been a strong rationale for developing genomic resources that can be used to increase the rate of genetic improvement for milk and meat production, disease resistance, feed efficiency and reproductive performance. Understanding the biology of cattle, particularly the unique features of ruminant metabolism [3], is a prerequisite for the sustainabil