The effects of GH and PDGF on the growth and proliferation of the epithelial cells

Introduction: Epithelial is an important tissue in our body. The human epithelium forms a barrierbetween the external and internal environment. Natural process of wound healing e.g. ischemia,necrotic tissues and infection is a concept that has been in practice for a long time. Large full thicknesswounds don’t heal spontaneously and are life treating. The closure of large wounds, when there islimited availability of autologous split skin grafts, has been a significant challenge to burns surgeonsover the last century.Material & Methods: Rat keratinocytes were isolated from pups 1 DIV and cultured according tothe to the protocol already described by Dulgos et al. Keratinocytes Isolated and were cultured inflask 75cm for 7 days ,then different concentrations of PDGF or GH were added daily for 6 days .Finally MTT test were done for all of wells(Case and Control) . Results were analyzed by Excel &SPSS.Results: Results of current study showed that keratinocytes culture were successfully established.There were two kinds of cells after 7 days in vitro. Both of the factors could stimulate cellsreproduction. Count of cells between concentrations of 10 – 200 ng of PDGF was significant(P<0.000). The concentration of 10ng of GH had the maximum effect in cells number and wassignificant (p<0.000). In comparison between two factors, the concentration of 100ng of PDGF wasthe best concentration for cells reproduction.Conclusion: These findings indicated that the extrinsic factors such as PDGF & GH could increasereproduction of keratinocyte cells in vitro