Production of Ethanol by Indigenous Wild and Mutant Strain of Thermotolerant Kluyveromyces Marxianus Under Optimized Fermentation Conditions

The maximum ethanol production and β-fructofuranocidase formation under fermentation studies were carried out in microprocessor controlled 23-L stainless steel fermenter at the following conditions: Temp = 40 C (wild &mutant organism) , pH= 5.5, carbon source (molasses=15% sugar) nitrogen source (ammonium sulphate 0.75%), 300 RPM stirring speed and oxygen flow rate was 0.1 vvm. Mutant. Strain of thermotolerant Kluyveromyces marxianus M15 produced maximum production of ethanol at 48hr.All Kinetic Parameters have been studied for the utilization of substrate and production of maximum ethanol for both wild and mutant strains. It has been observed that wild strain was growing up to 550C while the mutant strain was growing up to 650C. In this comparison study, wild and mutant strain showed that Mutant-derived M15 was stronger over its parental culture due to its more thermal stability and production of ethanol at 65 oC at which wild organism could not grow.