Antioxidative Activity of Extracts from Fenugreek Seeds (Trigonella foenum-graecum)

Spices and herbs possess antioxidant activity and can be applied for preservation of lipid peroxidation in biological systems. Fenugreek (Trigonella foenum-graecum) is an important spice; its dried seeds have wide application in food and beverages as a flavoring additive as well as in medicines. Crude extracts of fenugreek were prepared by soxhelt extraction method with different solvents such as methanol, ethanol, dichloromethane, acetone, hexane and ethyl acetate. Extracts were subjected for the measurement of total phenolic content (TPC) by Folin-Ciocalteu method as well as flavonoid content, chelating activity, reducing power and antioxidant/radical scavenging activity [1,1-diphenyl-2-picryl-hydrazyl (DPPH°) free radical scavenging activity]. Results from different parameters were in agreement with each other. The results reveal that all extracts of the fenugreek exhibit antioxidant activity. These findings suggest that the fenugreek extracts could act as potent source of antioxidants.