Plant regeneration from callus culture of vetiver (Vetiveria zizanioides Nash)

The present research aimed to establish cell suspension culture of vetiver (Vetiveria zizanioides Nash) from Surat Thani germplasm source and efficient plant regeneration from callus derived from such cultures. Cell suspension cultures were established from calli derived from inflorescence of vetiver. Optimum cell proliferation occurred in liquid N6 medium supplemented with 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 10 mM proline. The cell suspension formed the highest small colonies when plated on solid MS medium containing 0.45 μM 2,4-D. After subsequent transfer to regeneration medium (MS free medium) 65% of plantlets were obtained.