Analysis of genetic variation among Pummelo (Citrus maxima (Burm) Merrill) cv. Hom Hat Yai and indigenous in Songkhla province based on RAPD markers

RAPD technique was used to detect genetic variation among 85 samples of pummelo (Citrus maxima (Burm) Merrill) cv. Hom Hat Yai from different sites in Songkhla province. The genotypes analysed included 15 accessions of indigenous pummelo. DNA was exracted from leaf samples and 88 decamer primers were screened. Five primers that generated clear polymorphic fragments were chosen for further study. Identical banding patterns among all pummelo cv. Hom Hat Yai obtained in this study indicated no genetic variation. Banding patterns derived from 5 primers revealed significant differences between Hom Hat Yai and indigenous pummelos. The results of the pairwise analysis of the RAPD data indicated Hom Hat Yai showed a high similarity index with Sri-dok-kam and indigenous pummelos collected from Kuanlung district.