Development of medium for bacteriocin production from Lactobacillus casei ssp. rhamnosus SN 11

Coconut water and tuna condensate were used to produce bacteriocin from Lactobacillus casei ssp. rhamnosus SN 11 by comparing with commercial medium, MRS. Bacteriocin activity was determined by using Staphylococcus aureus as indicator. When L. casei ssp. rhamnosus SN 11 was grown in MRS medium the highest bacteriocin was observed at 18-24 hour with inhibitory activity of 20 AU/ml. While growing in modified media CW 1 (tuna condensate : coconut water = 1 : 1) the organism had inhibitory activity of bacteriocin of 20 AU/ml at 20 hour. In modified medium CW 2 (1 : 2), CW 3 (1 : 3), and CW 4 (1 : 4) hadinhibitory activity of bacteriocin of 10 AU/ml at 16 hour. The bacteriocin activity was also found when L. casei ssp. rhamnosus SN 11 grown in Tuna 2 (tuna condensate : coconut water = 2 : 1) and Tuna 3 (3 : 1) but no activity was observed in Tuna 4 (4 : 1).