Enhanced efficiency for propagation of anthurium by tissue culture technique

Enhanced efficiency for clonal propagation of anthurium could be carried out by increasing the concentration of adenine sulfate and overlaying liquid medium on solid medium. The results revealed that increasing concentration of adenine sulfate form 0.1 to 1 mg/l yielded an average number of shoot of 21 per explant. Addition of half strength liquid MS medium without phytohormone to the old cultures (agar solidified medium) gave optimum results in growth, healthy stem and leaves. In case of gelling agent, gelrite yielded a high number of shoots without callus formation. Proliferation rate of the shoots in gelrite solidified medium in the following subculture was not found. Thus, agar-agar was suitable for propagation in terms of low cost production and proliferation rate.