PREPARATION, CHARACTERIZATION AND EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF AN INTRAVENOUS LIPOSOMAL FORMULATION OF BIS-DEMETHOXY CURCUMIN ANALOGUE (BDMCA)

The aim of study was to prepare small unilamellar vesicles (SUVs) incorporating BDMCA that can injected by intravenousroute and further, evaluate hepatoprotective activity of the formulation. SUV liposomes were prepared using thin filmhydration followed by sonication method. Soya lecithin was used as lipid and stearyl amine was used as cationic chargeinducer. In the preparation of liposomes, process and formulation parameters were standardized. After preparation SUVswere characterized for physicochemical properties, particle size, zetapotential, percent drug entrapment, in vitro drugrelease and the drug-polymer interaction. The sustenance of drug release into the plasma after intravenous BDMCA SUVadministration was determined. Hepatoprotective activity was evaluated in CCl4 treated rats. The liposomal formulationswere successfully prepared using thin film hydration followed by sonication method. The desired encapsulation wasachieved by increase in the area of the lipid film formed. The size of SUVs obtained was 327 nm. FTIR results indicate therewas no interaction between lipid and drug. In vitro release data showed that the release was sustained for 10 days in vitroand could be described as diffusion-controlled. The liposomal formulations were able to sustain the release of drug in vivoalso. Liposomal formulations showed better hepatoprotective activity to the drug compared to its solution form.