Effect of Glycerol-Induced Hyperhydration on Body Fluid and Electrolyte Balance in Endurance Athletes during The Course of Treadmill Exercise Performed at 30 °C for 90 minute

The purpose of this study was to determine the effects of glycerol-induced hyperhydration on body fluid and electrolyte balancein endurance athletes during the course of treadmill exercise performed at 30 C for 90min. 9 elit level male long-distance runnerwere participated to this study (age: x = 18,7 ±1,3 years, height: x = 170,7±5,2 cm, body weight: x = 58,8±6,6 kg, VO2max:63,94±3,04 ml.kg-1). First, VO2max of the subjects were determined with an incremental treadmill running protocol. In a randomized,double-blind cross over experimental design subjects were tested three times with 3 days intervals (wash out) following ingestion of20 ml.kg-1BW of three different mixture of solutions: 1) diluted sports drink with 1.2 gr.kg-1BW glycerol (GS) 2) diluted sports drink(SP) and 3) aspartame flavored distilled water (WS). Exercise trials were conducted at an exercise intensity of 65% maximal oxygenconsumption (VO2max) for 90 min at 30±1.8 C and 25-35% relative humidity. Blood and urin samples were collected pre and postfluid ingestion, at the 30th, 60th and 90th min of exercise trials to determine body fluid and electrolyte balance. Data were analyzedusing two-way (treatmentxtime) analyses of variance (ANOVA). Significance level was defined as p<0.05. No significant differencewas found among the three solutions ingested with respect to their effects on fluid and electrolyte parameters (p>0.05). Inconclusion, glycerol-induced hyperhydration has no advantage compared to the other solutions ingested on body fluid andelectrolyte balance in endurance athletes during 90 min of treadmill run.