Efecto del control biológico por antagonistas sobre fitopatógenos en vegetales de consumo humano

Taking in account the negative actions of pesticides exert on natural environment and health and due to the possibility to minimize/eliminate its use, the effect of microbial antagonist (Trichoderma viride, Pseudomonas aeruginosa and Bacillus subtilis), were evaluated on tomato, onion and pimento seeds experimentally infected with three known phytopatogens (Phytophthora spp, Fusarium monilliforme and Rhyzoctonia solani). Initially, the antagonist effect on the phytopatogens were studied on Sabouraud solid (agar) and liquid (broth) media. Further 35 seeds of each vegetable were treated according to the following experimented protocol group I (control): no addition and water, group II (treated): antagonist and water, group III (infected): antagonist, water and phytopathogens. All groups were grown on soil, under sun light, at room temperature and water was added twice a day. Group III was daily infected with 0,1 mL of the antagonist suspensions (104 UFC/mL). The plant development was followed for 2 weeks, determining number of germinated seeds (germination index), plantule length and foliage. The results obtained was analyzed with Statistix 8.0, Tukey and Kruskal-Wallis. The antagonist inhibited the proliferation of the phytopathogens in Sabouraud media. The seed treated and infected showed a higher index of germination and plantule respect to the control and treated groups (p<0,1)