Fabrication of docetaxel surfaced Fe3O4 magnetite nanoparticles and their cytotoxicity on 4？T1 breast cancer cells

Fe3O4 MNPs were synthesised by a chemical method and characterized by transmission electron microscopy and energy-dispersive spectroscopy techniques. In the next step, docetaxel-coated Fe3O4 MNPs were prepared, using percipitation method. The surface chemistry of docetaxel-coated Fe3O4 MNPs as well as their thermal decomposition characteristics were examined using fourier transform infrared spectroscopy and thermogravimetric analyzer equipment, respectively. The cytotoxicity assay was conducted on 4？T1 breast cancer carsinoma by MTT assay to evaluate the possible in vitro antiproliferative effects of docetaxel-coated Fe3O4 MNPs.During precipitation process, docetaxel molecules were precipitated on the surface of Fe3O4 MNPs by the ratio of 3:100 w/w which indicates that each milligram of coated Fe3O4 MNPs averagely contained 30？μg pure docetaxel compound. Docetaxel showed aniproliferative effects against mentioned cell line. The higestest concentartion of docetaxel (80？μg/ml) caused about 80% cell death. However, the results demostarted that much lower amounts of docetaxel will be needed in combination of Fe3O4 MNPs to produce the potent antiproliferative effect compared to docetaxel alone. Dose response cytotoxicity assay of docetaxel-coated Fe3O4 MNPs against 4？T1 breast cancer cells showed that lower amount of docetaxel (0.6？μg/ml) can exhibit higher cytotoxic effect against this cancer cell line (90% cell death).Today, side effects of anti cancer drugs are still considered as a major problem in chemotherapy of cancer diseases ([1]). In the recent years, new drug delivery systems have been developed to reduce the side effects of these drugs ([2-4]). These systems mainly include nanotubes ([5]), liposomes ([6]), dendrimers ([7]) and nanoparticles ([8]). The potential biomedical applications of Fe3O4 magnetite nanoparticles (MNPs) are well discussed in the literature ([9]). Due to their magnetic properties, these nanomaretials have recieved particular attention as p