Comparative in vitro study of the antimicrobial activities of different commercial antibiotic products of vancomycin

Microbial assays were used to establish the potency, the Minimal Inhibitory Concentrations (MICs), the Minimal Bactericidal Concentration (MBCs), the critical concentrations, and the production of spontaneous mutants that are resistant to vancomycin.The microbial assay was validated in order to determine the Vancomycin potency of the tasted samples. All the products showed that have potency values between 90 - 115% (USP requirement). The products behave similarly because the MICs, The MBCs, the critical concentrations, the critical concentrations ratios between standard and samples, and the production of spontaneous mutants don't have significant differences.All products analyzed by microbiological tests, show that both trademarks and generics do not have statistical variability and the answer of antimicrobial activity Show also that they are pharmaceutical equivalents.Pharmaceutical products, especially antibiotics, must comply with standards of quality, efficacy and reliability, attributes that are determined by various authorities [[1,2], and [3]]. A discussion about the quality and efficacy of generic antibiotics has taken place in recent decades. This discussion has included presentations in congress and research articles in which the authors have shown that some products do not meet regulatory standards [4,5] and that their behavior is not similar in animal models [6,7]Some antibiotics must be analyzed using biological assays (e.g., penicillin, amikacyn, vancomycin, and neomycin) [2]. These products are measured by their potency or biological activity compared against an international standard. Therefore, the commercial products must be similar in composition to the international reference standard [7]. With antibiotics like vancomycin, if the commercial products do not fulfill the requirements of pharmacopeia, their behavior and performance could put a patient's health in danger.Biological assays and other analytical procedures must be validated before they a