Homocysteine levels in preterm infants: is there an association with intraventricular hemorrhage? a prospective cohort study

123 infants < 32 weeks gestation admitted to our Level III nursery were enrolled. A group of 25 term infants were enrolled for comparison. Two blood spots collected on filter paper with admission blood drawing were analyzed by a high performance liquid chromatography (HPLC) method. Statistical analysis included ANOVA, Spearman's Rank Order Correlation and Mann-Whitney U test.The median tHcy was 2.75 μmol/L with an interquartile range of 1.34 – 4.96 μmol/L. There was no difference between preterm and term tHcy (median 2.76, IQR 1.25 – 4.8 μmol/L vs median 2.54, IQR 1.55 – 7.85 μmol/L, p = 0.07). There was no statistically significant difference in tHcy in 31 preterm infants with IVH compared to infants without IVH (median 1.96, IQR 1.09 – 4.35 μmol/L vs median 2.96, IQR 1.51 – 4.84 μmol/L, p = 0.43). There was also no statistically significant difference in tHcy in 7 infants with periventricular leukomalacia (PVL) compared to infants without PVL (median 1.55, IQR 0.25 – 3.45 μmol/L vs median 2.85, IQR 1.34 – 4.82 μmol/L, p = 0.07). Male infants had lower tHcy compared to female; prenatal steroids were associated with a higher tHcy.In our population of preterm infants, there is no association between IVH and tHcy. Male gender, prenatal steroids and preeclampsia were associated with differences in tHcy levels.Intraventricular hemorrhage (IVH) and periventricular leukomalacia (PVL) are significant causes of morbidity and mortality in the preterm infant population, yet aspects of the multifactorial pathophysiology leading to these CNS insults remain unclear. Petaja et al has suggested that thrombophilia may play a role in the etiology of IVH in preterm infants [1]. They identified 32% of infants with IVH as having abnormal prothrombotic factors [1]. Others have implicated activated coagulation factors in cerebral white matter damage; these activated factors are thought to cause injury by exacerbating inflammation rather than by occlusion of cerebral vessels [2]. Addition