Stallion spermatozoa selected by single layer centrifugation are capable of fertilization after storage for up to 96？h at 6°C prior to artificial insemination

Sixteen mares were inseminated with SLC-selected sperm doses that had been cooled and stored at 6°C for 48？h, 72？h or 96？h. Embryos were identified in 11 mares by ultrasound examination 16–18？days after presumed ovulation.SLC-selected stallion spermatozoa stored for up to 96？h are capable of fertilization.