Separation and Determination of Morphine, Penthidine and Diazepam by High Performance Liquid Chromatography
高效液相色谱法对吗啡、杜冷丁、安定的同步测定

Morphine, penthidine and diazepam in blood were determined by high performance liquid chromatography (HPLC). The HPLC system was consisted of 10 microns particle size C18 column and UV detector set at 254 nm. The mobile phase was V(CH3OH):V(25 mmol/L KH2PO4) = 90:10 with a flow rate of 1.0 mL/min. The average recoveries of morphine, penthidine and diazepam were 93.27%, 90.23% and 93.29% respectively. The linear range was between 0.05-50 mg/L. The detection limit was 0.05 mg/L and CV was less than 6%.