全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
色谱  2000 

Determination of EDTA Residue in Canned Food Products by RP-HPLC
反相高效液相色谱法测定罐头食品中乙二胺四乙酸的残留量

Keywords: high performance liquid chromatography,ethylenediaminetetra,acetic acid,(EDTA),canned food
乙二胺四乙酸
,罐头食品,残留量分析,HPLC

Full-Text   Cite this paper   Add to My Lib

Abstract:

The homogenized sample is diluted with water. After adding copper(II) chloride solution and 20 mg of ascorbic acid, dilute the sample to the volume with water. After thorough mixing and microfiltration over a 0.45 microns filter, the filtrate is ready for injection into the HPLC system and detected at 254 nm. The 125 mm x 4 mm i.d. chromatographic column is packed with Hypersil ODS 5 microns, eluted with a mobile phase of water-methanol (80:20, V/V) containing 20 mmol/L tetrabutyl ammonium bromide, 0.03 mol/L sodium acetate buffer (pH 4) at a flow rate of 0.8 mL/min. The injection volume is 20 microL. The response value was linear between 10 mg/L-400 mg/L. The recovery was 95.5%-98.9%. The RSD was 0.82%-1.32%.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133