Application of on-line two-dimensional liquid chromatography in comparative proteome analysis of antlers with different growing stages
基于在线二维色谱的不同生长时期鹿茸的比较蛋白质组分析

A method of on-line two-dimensional weak anion exchange chromatography-reversed-phase liquid chromatography(2D WAX-RPLC) was established,and applied for the comparative proteome study of antlers with four different growing stages.A five-protein-mixture was used as the sample to evaluate the reproducibility of the system.In addition,the capacity of such a system for the relative quantitative analysis was demonstrated by analyzing protein mixtures with different concentration ratios.Furthermore,five different...