Thermodynamic characteristics of stoichiometric displacement linear parameter log I in reversed-phase liquid chromatography]
反相液相色谱中计量置换线性参数logI的热力学特征

Based on the physical meaning of each term in the linear parameter log I (a constant correlating the affinity of solute to stationary phase) of stoichiometric displacement model for retention(SDM-R) of solute, the thermodynamic characteristics of log I of solute in reversed-phase liquid chromatography (RPLC) were investigated theoretically. This point was tested by experimental data with two linear relationships: (1) plot of log I vs 1/T (T is absolute temperature) with apolar, polar small solutes and proteins; (2) plot of log I vs log Po/w (partition coefficient of solute between n-octanol and water) with apolar and polar small solutes. From the comparison of the magnitude of partition coefficient between small solute and proteins, a phenomenon that the resolution of small solutes depends on column length but that of proteins almost to be independent of column length is quantitatively explained.