Determination of Sulfacetamide Sodium and Sulfanilamide in Shao Tang Ling Ointment by High Performance Liquid Chromatograpby
高效液相色谱法测定烧烫灵软膏中磺胺醋酰钠和磺胺含量

An HPLC method for the determination of sulfacetamide sodium and sulfanilamide in Shao Tang Ling ointment was developed. The chromatographic conditions were: column, Spherisorb C18, mobile phase,methanol-0. 25% (V/V)-acetic acid solution (7: 93) ; detector UV-257 nm. The average recoveries were 98.9% (n= 5), RSD= 0. 64% for sulfacetamide sodium and 99. 3 % (n = 5), RSD= 1. 58% for sulfanilamide.The analytical method is simple, quick, accurate and reproducible.