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色谱  2004 

Study on Subcritical Water Extraction of Baicalin from Radix Scutellariae Coupled to High Performance Liquid Chromatographic Analysis
黄芩中黄芩苷的亚临界水提取及高效液相色谱分析

Keywords: subcritical water extraction,high performance liquid chromatography,baicalin,Chinese traditional medicine,Radix Scutellariae
黄芩
,黄芩苷,亚临界水提取,高效液相色谱分析,实时监测,脂溶性成分,中药材

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Abstract:

A method for the extraction of baicalin from Radix Scutellariae with subcritical water coupled to high performance liquid chromatographic analysis was established. The effects of various parameters, which included the pressure, temperature, extraction time, and the particle size of the plant material as well as the solvent/sample ratio on the yield were investigated. Compared to the conventional organic solvent extraction method, the subcritical water extraction method showed shorter handling time and lower solvent consumption without waste producing. The optimal conditions of extraction of baicalin from Radix Scutellariae with subcritical water were 0.15 - 0.18 mm particle size, solvent/sample ratio of 0.2 mL/mg, 5 MPa, 130 degrees C for about 10 min. This technique seems very promising for the extraction of liposoluble substances from plant materials.

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