The Effect of Mobile Phase on the Enantiomeric Separation of Cyfluthrin by Chiral High Performance Liquid Chromatography
流动相对百树菊酯高效液相色谱手性分离的影响

The enantiomeric separation of cynuthrin on chiral stationary phase (CSP), derived from (R)-N(3, 5-dinitrobenzoyl) phenylglycine (Prikle type 1-A CSP) has been studied by using different he-cane-alcohol mixtures as mobile phase. Mobile phase strength and composition have shown an important role in the chromatographic separation of eight isomers of cyfluthrin. Increase of the mobile phase strength reduces the retention of the eight isomers, while the diastereomeric selectivity is decreased and the enantiomeric selectivity remains almost unchanged. Also, the resolution varies in accordance with the steric hindrance of the alcohol.Secondary or tertiary alcohols impart a greater resolution to the CSP than primary alcohols. Peak shapes are better with the lower alcohols than the higher alcohols. For the enantiomeric separation of cyfluthrin on Pirkle type 1-A CSP, hexane-tert-butanol and hexane-2-propanol are better binary mobile phases.