Isolation and purification of esculetin from the seeds of Euphorbia lathyris L. using high-speed counter-current chromatography
高速逆流色谱法分离纯化续随子种子中的七叶内酯

A method for the isolation and purification of esculetin from the seeds of Euphorbia lathyris L. was developed using high-speed counter-current chromatography (HSCCC). The ethyl acetate extract of the seeds of Euphorbia lathyris L. was separated by the HSCCC directly. Different solvent systems were investigated, and the results showed that the best solvent system was the two-phase solvent system composed of chloroform-methanol-water (4:3:2, v/v/v). The lower phase was used as the mobile phase and the upper phase was used as the stationary phase. A total of 80 mg esculetin with the purity of 99.04% was successfully obtained from 200 mg crude extract of the seeds of Euphorbia lathyris L. The results indicate that optimized HSCCC offers a preferred method for the preparation of esculetin from the seeds of Euphorbia lathyris L.