Preparation of Immobilized Metal-Chelated Affinity Membrane and Its Application to Purification of Cu/Zn-Superoxide Dismutase
固定化金属螯合亲和膜色谱柱制备及纯化铜锌超氧化物歧化酶的研究

Immobilized metal-chelated affinity membranes were prepared using macropore cellulose filter paper as matrix. The matrix was treated with alkaline, activated with epichlorohydrin and coupled with iminodiacetate sodium, and then Cu2+ was immobilized. Cu/Zn-superoxide dismutase(SOD) was purified with prepared affinity membranes. The special activity of the purified Cu/Zn-SOD was increased 10.7 times from 645 U/mL to 6,882 U/mL. The protein recovery and the activity recovery were 92.3% and 985%, respectively. A new method to prevent the leaking of metal ion was developed and Cu2+ concentration in the effluent was lowered to 86 micrograms/L.