Determination of robenidine residue in chicken tissues and eggs by high performance liquid chromatography
高效液相色谱法测定鸡组织与鸡蛋中氯苯胍的残留量

A method for the determination of robenidine residue in chicken tissues and eggs by high performance liquid chromatography (HPLC) was established. The samples were extracted with acetonitrile and purified by an HLB solid-phase extraction (SPE) cartridge. The extract was analyzed by HPLC with acetonitrile-0.05 mol/L NH4H2PO4 buffer (6:4, v/v, pH 6.5) as the mobile phase. The flow rate of the mobile phase was 1.0 mL/min and detection wavelength was 317 nm. There was a good linear correlation between the peak areas and the concentrations of robenidine in the range of 10~1000 μg/L. The limit of detection (S/N=3) was 10 μg/L, and the limit of quantification (S/N=10) was 15 μg/kg. The recoveries of robenidine were 73.1%~88.7% at the spiked levels of 15, 50 and 100 μg/kg. The results demonstrate that the method is easy, fast, sensitive, and suitable for the confirmation and quantification of robenidine residue in chicken tissues and eggs. The clean-up effect, sensitivity, accuracy and precision can all meet the analysis requirement.