Detection of Residual Antibiotics in Honey by Capillary High Performance Liquid Chromatography
蜂蜜中残留抗生素的填充毛细管液相色谱法检测

The quantitative analysis of the three antibiotics-tetracycline(TC), oxytetracycline(OTC) and chlortetracyclinum(CTC) was carried out with a self-packed Hypersil C18 150 mm x 0.5 mm i.d. column. As little as 0.5 nanogram of antibiotics can be well detected, the capillary high performance liquid chromatographic method is 100 times more sensitive than that with ordinary HPLC method. The coefficients of linear correlation of mass concentration from 0.5 nanogram to 20 nanogram were rOTC = 0.99695, rTC = 0.99778 and rOTC = 0.98836. It showed that capillary HPLC is a good method used for sensitive analysis of antibiotics in honey.