Rapid analysis of trace levels of riboflavin and its derivatives with hydrophilic interaction monolith by pressurized capillary electrochromatography with laser induced fluorescence detection
亲水作用加压毛细管电色谱-激光诱导荧光检测法分析痕量核黄素类物质

A novel hydrophilic interaction capillary electrochromatographic(HI-CEC) monolith with covalently bonded zwitterionic functional groups was applied for the separation of riboflavins(RF) and its derivatives.With a homemade pressurized capillary electrochromatography-laser induced fluorescence detection(pCEC-LIF) system,trace levels of RF and its derivatives,flavin mononucleotide(FMN) and flavin adenine dinucleotide(FAD),can be baseline separated within 8.0 min in isocratic elution mode.The effect of experime...