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色谱  2010 

Determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography with evaporative light scattering detection
高效液相色谱-蒸发光散射检测法测定番茄中的番茄皂苷A

Keywords: high performance liquid chromatography (HPLC),evaporative light scattering detection (ELSD),esculeoside A,Lycopersicon esculentum MILL
高效液相色谱法
,蒸发光散射检测,番茄皂苷A,番茄

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Abstract:

A method for the determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) was established. The results showed that esculeoside A had good linearity in the range of 0.61~3.05 mg with the correlation coefficient of 0.9995. The average recoveries were 97.9%~104.8% with the relative standard deviations (RSD) ≤4.14% (n=5). The method is simple, sensitive and suitable for the analysis of esculeoside A and quality control for the raw material and extract of Lycopersicon esculentum MILL.

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