|
色谱 2010
Determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography with evaporative light scattering detection
|
Abstract:
A method for the determination of esculeoside A in Lycopersicon esculentum MILL by high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) was established. The results showed that esculeoside A had good linearity in the range of 0.61~3.05 mg with the correlation coefficient of 0.9995. The average recoveries were 97.9%~104.8% with the relative standard deviations (RSD) ≤4.14% (n=5). The method is simple, sensitive and suitable for the analysis of esculeoside A and quality control for the raw material and extract of Lycopersicon esculentum MILL.