Simultaneous Determination of Trace Ni2+, Mn2+, Zn2+ and Cu2+ with Microwave Derivatization/Ion-Pair High Performance Liquid Chromatography/Meso-Tetra(4-Sulfophenyl)-Porphyrin Spectroscopy
微波衍生-离子对高效液相-磺化四苯基卟啉光度法同时测定痕量镍、铜、锰和锌

The best conditions for the reaction of meso-tetra(4-sulfophenyl)-porphyrin (TPPS4) with Mn2+, Ni2+, Zn2+ and Cu2+ have been investigated. A method for the fast determination of Ni2+, Mn2+, Zn2+ and Cu2+ has been developed: microwave derivatization at 750 W for 3 min, C18 column, acetonitrile-water(22.5:77.5, volume ratio) as mobile phase with tetraethylammonium bromide (TEABr) as ion-pair reagent and detection wavelength of 415 nm. The detection limits for Zn2+, Cu2+, Ni2+ and Mn2+ were 0.05 microgram/L, 0.01 microgram/L, 0.10 microgram/L and 0.40 microgram/L, respectively. This method has been applied to analyze the concentrations of these four metal ions in marketed tea.