Determination of Fatty Acids in Shark Cartilage by GC-MS Using Microwave-Assisted Digestion and Derivatization
微波消解衍生化气相色谱-质谱法测定鲨鱼软骨中的脂肪酸

A rapid microwave-assisted digestion and derivatization method for the determination of fatty acids in shark cartilage by GC-MS was developed. The optimum conditions for digestion and derivatization were studied in detail using orthogonal design. The digestion and derivatization were accomplished in 4 minutes at 600 W microwave power using HCl-methanol (1:4, V/V) as digestion and derivatization solvent, and the extraction of the target analytes could be carried out simultaneously. This method is rapid, solvent-saving, and particularly suitable for the rapid determination of fatty acids in solid samples.