Determination of Atrazine and Its Metabolites in Human Urines Using Gas Chromatography
气相色谱法分析尿液样品中的阿特拉津及其代谢物

Atrazine(ATZ) was widely used to control broadleaf weeds.Numerous animal experiments have proved that atrazine is a suspicious endocrine disruptor.Thus,further development of the ability to estimate low-dose human exposure to atrazine is requested in epidemiologic studies to correlate the toxicological effects associated with the concentrations of ATZ and its metabolites in human body.A method for detecting ATZ and its metabolites(deethylatrazine(DEA),deisopropylatrazine(DIA),deethyldeisopropylatrazine(DEDIA)) in human urines using gas chromatography was established.A urine sample was extracted by ethyl acetate,and purified using a Florisil column.Final concentrated extract was analyzed by a gas chromatograph-electron capture detector.The conditions of this method were optimized.The limits of detection were 0.002 5 mg/L for DEDIA,0.005 mg/L for DEA,DIA and ATZ.The linear ranges were from 0.2 to 8 ng for all analytes.The atrazine concentrations in urine samples of the workers collected from an atrazine plant were determined by this method.The concentration ranges were 0.003-0.301 mg/L for DEDIA,0.005-0.011 mg/L for DEA,0.006-0.276 mg/L for DIA,and 0.005-0.012 mg/L for ATZ.