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色谱  2006 

Study on the Fingerprints of Fructus Forsythiae by High Performance Capillary Electrophoresis
连翘的毛细管电泳指纹图谱研究

Keywords: capillary electrophoresis(CE),Fructus Forsythiae,fingerprints,information(index,) relative information index
毛细管电泳法
,连翘,指纹图谱,信息量指数,相对信息量指数

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Abstract:

The capillary electrophoresis fingerprints(CEFP) of Fructus Forsythiae was established to control its quality.The background electrolyte(BGE) was a 75 mmol/L sodium borate solution adjusted to pH 9.7 with 0.1 mmol/L NaOH solution.The detection wavelength was 228 nm and a voltage of 14 kV was applied.The Forsythia suspensa(Thunb.) Vahl was extracted by water and a set of CEFP containing 29 peaks was obtained.The CEFP had acceptable precision and reproducibility with the relative standard deviation(RSD) of the relative migration time less than 5.0% and the RSD of the relative peak area from 2% to 15%.There were good similarities between the standard CEFP and each set of CEFP of Forsythia suspensa(Thunb.) Vahl from ten different places,and their similarity coefficients were not less than 0.94.The CEFP were also evaluated by the information index I and the relative(information index) I_r of chromatographic fingerprint.

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